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首页> 外文OA文献 >Pyrosequencing andde novoassembly of Antarctic krill (Euphausia superba)transcriptome to study the adaptability of krill to climate-induced environmental changes
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Pyrosequencing andde novoassembly of Antarctic krill (Euphausia superba)transcriptome to study the adaptability of krill to climate-induced environmental changes

机译：南极磷虾转录组的焦磷酸测序和从头组装研究磷虾对气候引起的环境变化的适应性

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The Antarctic krill, Euphausia superba, has a key position in the Southern Ocean food web by serving as direct link between primary producers and apex predators. The south-west Atlantic sector of the Southern Ocean, where the majority of the krill population is located, is experiencing one of the most profound environmental changes worldwide. Up to now, we have only cursory information about krill's genomic plasticity to cope with the ongoing environmental changes induced by anthropogenic CO2 emission. The genome of krill is not yet available due to its large size (about 48 Gbp). Here, we present two cDNA normalized libraries from whole krill and krill heads sampled in different seasons that were combined with two data sets of krill transcriptome projects, already published, to produce the first knowledgebase krill 'master' transcriptome. The new library produced 25% more E. superba transcripts and now includes nearly all the enzymes involved in the primary oxidative metabolism (Glycolysis, Krebs cycle and oxidative phosphorylation) as well as all genes involved in glycogenesis, glycogen breakdown, gluconeogenesis, fatty acid synthesis and fatty acids β-oxidation. With these features, the 'master' transcriptome provides the most complete picture of metabolic pathways in Antarctic krill and will provide a major resource for future physiological and molecular studies. This will be particularly valuable for characterizing the molecular networks that respond to stressors caused by the anthropogenic CO2 emissions and krill's capacity to cope with the ongoing environmental changes in the Atlantic sector of the Southern Ocean.
机译：南极磷虾Euphausia superba在主要海洋生产者和先端捕食者之间具有直接联系，在南方海洋食物网中占有重要地位。磷虾种群最多的地区位于南大西洋的西南大西洋地区，正在经历着全球范围内最深刻的环境变化之一。到现在为止，我们只有关于磷虾的基因组可塑性的粗略信息，以应对人为二氧化碳排放引起的持续的环境变化。磷虾的基因组由于其较大的大小（约48 Gbp）而无法获得。在这里，我们介绍了两个从整个磷虾和不同季节采样的磷虾头部归一化的cDNA文库，这些文库与已经发布的两个磷虾转录组项目数据集组合在一起，以产生第一个知识库磷虾“主”转录组。新文库产生的超级大肠杆菌转录产物增加了25％，现在几乎包括了参与初级氧化代谢的所有酶（糖酵解，克雷布斯循环和氧化磷酸化），以及涉及糖生成，糖原分解，糖异生，脂肪酸合成的所有基因。和脂肪酸β-氧化。具有这些功能的“主”转录组可提供南极磷虾代谢途径的最完整信息，并为将来的生理和分子研究提供重要资源。这对于表征响应人为二氧化碳排放和南极磷虾应对南大洋大西洋地区环境变化的能力的应激源的分子网络特别有价值。

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